The tTA atlas is an interactive resource providing access to brain wide histological images displaying the distribution patterns of promoter activity in different tetracycline transactivator (tTA) mouse models

Experimental procedures:

For details, please cf. Yetman et al., 2015.

Animals:

  • adult 4-6 month old bigenic Nop-tTA/tetO-lacZ-nls-GFP mice
  • adult 4-6 month old single transgenic tetO-lacZ-nls-GFP mice (controls)

Immunohistochemistry:

  • Sagittal sections from right hemisphere were cryosectioned at 40 µm.
  • β-galactosidase was labeled blue using X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) as a substrate
  • Weak counterstaining with Neutral Nuclear Fast Red facilitated detection of boundaries and landmarks without compromising the observation of X-gal labeling

Slidescanning:

  • Digital images were created using a slidescanner (AxioScan Z1, Carl Zeiss Microimaging, Jena, Germany)

 

Overview

The atlas contains histological data documenting the activity of the Nop-tTA promoter in bigenic mice in which the Nop-tTA promoter regulates the expression of the LacZ reporter gene, encoding the histologically detectible marker β-galactosidase.

The Virtual Microscope tool allows users to navigate within and across section images.

The atlas is based on data from the following publication:

Yetman MJ, Lillehaug S, Bjaalie JG, Leergaard TB, Jankowsky JL (2015), Transgene expression in the Nop-TTA driver line is not inherently restricted to the entorhinal cortex. Brain Struct Funct DOI 10.1007/s00429-015-1040-9 (accepted manuscript)

Re-use of data from this repository is permitted provided reference is made to this publication. For methodological details, cfr. Yetman et al. (2015).